September 1, 2000
- Eritrea
- Revolution Day
- Slovakia
- Constitution Day
- Uzbekistan
- Independence Day
- Iran
- Martyrdom of Fatima-Zahara
September 3, 2000
- San Marino
- The Feast of San Marino and the Republic
September 4, 2000
- American Samoa
- Labour Day
- Bermuda
- Labour Day
- Canada
- Labour Day
- Guam
- Labour Day
- Puerto Rico
- Labour Day
- Swaziland
- Umhlanga Reed Dance
- United States of America
- Labour Day
- U.S. Virgin Islands
- Labour Day
September 5, 2000
- Brazil
- Elevação do Amazonas à categoria de província
September 6, 2000
- Bulgaria
- Unification Day
- Caribbean Netherlands
- Bonaire Day
- São Tomé & Príncipe
- Armed Forces Day
- Swaziland
- Independence Day
September 7, 2000
- Brazil
- Independence Day
- Switzerland
- Jeûne Genevois
- Spain
- La Bajada de la Virgen del Socorro
- Fiji
- Constitution Day
- Canary Islands
- La Bajada de la Virgen del Socorro
- Mozambique
- Lusaka Peace Agreement
September 8, 2000
- Andorra
- Our Lady of Meritxell
- Brazil
- Nossa Senhora da Luz dos Pinhais (PR)
- Nossa Senhora da Natividade (TO)
- Spain
- Día de Asturias (AS)
- Nuestra Señora del Pino (CN)
- Día de Extremadura (EX)
- Canary Islands
- Nuestra Señora del Pino
- Lichtenstein
- Mariä Geburt
- Macedonia
- Independence Day
- Malta
- Victory Day
- Vatican City
- Nativity of Mary
September 12, 2000
- China
- Mid-Autumn Festival
- Cape Verde
- National Day
- Eritrea
- Geez New Year
- Ethiopia
- New Year's Day
- South Korea
- Korean Thanksgiving
- Taiwan
- Mid-Autumn Festival
- United States of America
- Primary Election Day
September 15, 2000
- Costa Rica
- Independence Day
- Spain
- La Bien Aparecida (CB)
- Nuestra Señora de los Dolores (CN)
- Guatemala
- Independence Day
- Honduras
- Independence Day
- Canary Islands
- Nuestra Señora de los Dolores
- Japan
- Respect-for-the-Aged Day
- Nicaragua
- Independence Day
- Slovakia
- Day of Our Lady of the Seven Sorrows
- El Salvador
- Independence Day
September 16, 2000
- Brazil
- Emancipação política
- St. Kitts & Nevis
- National Heroes' Day
- Libya
- Martyrs' Day
- Mexico
- Independence Day
- Malaysia
- Malaysia Day
- Papua New Guinea
- Independence Day
September 17, 2000
- Angola
- National Hero Day
- Switzerland
- Federal Day of Thanksgiving, Repentance and Prayer
September 18, 2000
- Chile
- National holiday
- Spain
- Nuestra Señora de la Peña
- Canary Islands
- Nuestra Señora de la Peña
- Tonga
- Birthday of His Royal Highness The Crown Prince Tupotoʻa-ʻUlukalala
September 19, 2000
- Chile
- Army Day
- St. Kitts & Nevis
- Independence Day
September 20, 2000
- Brazil
- Proclamação da República Rio-Grandense
September 22, 2000
- Bulgaria
- Independence Day
- Switzerland
- Mauritiustag
- Republic of Mali
- Independence Day
- Papua New Guinea
- September Equinox
September 23, 2000
- Japan
- Autumnal Equinox Day
September 24, 2000
- Austria
- Rupert
- Bolivia
- Pando Day (N)
- Santa Cruz Day (S)
- Dominican Republic
- Our Lady of las Mercedes
- Spain
- Nuestra Señora de los Reyes
- Guinea-Bissau
- Independence Day
- Canary Islands
- Nuestra Señora de los Reyes
- New Caledonia
- New Caledonia Day
- Trinidad & Tobago
- Republic Day
- South Africa
- Heritage Day
- Cambodia
- Constitution Day
September 25, 2000
- Australia
- Queen's Birthday
- Bolivia
- Pando Day (substitutes) (N)
- Santa Cruz Day (substitutes) (S)
- Switzerland
- Saint Nicholas of Flüe
- Spain
- Mare de Déu de la Mercè
- Mozambique
- Defense Force’s Day
- Trinidad & Tobago
- Republic Day (substitute day)
- South Africa
- Public Holiday
September 27, 2000
- Cambodia
- Pchum Ben Day
September 28, 2000
- Czech Republic
- St. Wenceslas Day (Czech Statehood Day)
- Eritrea
- Meskel
- Ethiopia
- Finding of the True Cross
- Cambodia
- Pchum Ben Day
September 29, 2000
- Australia
- AFL Grand Final Friday
- Paraguay
- Boqueron Battle Victory Day
- São Tomé & Príncipe
- Agricultural Reform Day (substitute day)
- Turks & Caicos Islands
- National Youth Day
September 30, 2000
- Botswana
- Botswana Day
- São Tomé & Príncipe
- Agricultural Reform Day
- Israel
- Rosh Hashanah (Yom Teruah)
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